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Microsatellites as a powerful tool for identification of olive (Olea europaea L.) planting material in nurseries
Dunja Bandelj, Branka Javornik, 2007, original scientific article

Abstract: Microsatellites were applied to confirm the varietal identity of thirteen olive samples from a private nursery. Allelic profiles of samples were compared to genotyped reference varieties from three olive germplasm collections. Genotyping data of two microsatellite loci (ssrOeUA-DCA3 and ssrOeUA-DCA16) allowed discrimination of ten olive samples and the identification of six olive varieties (Koreneiki, Manaki, Tsounati, Kalamon, Ascolana Tenera, Picual). Mislabelling of two olive accessions (Manyanilla de Sevilla, Coratina) was observed and the lack of reference varieties Nostrana Bitontina and Miloelia hindered the identification of two accessions from the nursery. Accessions denominated as Miloelia and Gaiduroelia were of identical genotype. Different allelic profiles of the Frantoio reference variety were observed from two national olive germplasm collections. Microsatellites were found to be a valuable tool for varietal identity confirmation, in nurseries as well as for screening and managing olive germplasm collections.
Keywords: Olea europaea L., microsatellites, identification, variety
Published in RUP: 10.07.2015; Views: 3514; Downloads: 28
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