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Title:Neural agrin changes the electrical properties of developing human skeletal muscle cells
Authors:Jurdana, Mihaela (Author)
Fumagalli, Guido (Author)
Grubič, Zoran (Author)
Lorenzon, Paola (Author)
Marš, Tomaž (Author)
Sciancalepore, Marina (Author)
Files:URL http://www.springerlink.com/content/kgt25366m3721011/fulltext.pdf
 
Language:English
Work type:Not categorized
Tipology:1.01 - Original Scientific Article
Organization:UPR - University of Primorska
Abstract:Recent investigations suggest that the effects of neural agrin might not be limited to neuromuscular junction formation and maintenance and that other aspects of muscle development might be promoted by agrin. Here we tested the hypothesis that agrin induces a change in the excitability properties in primary cultures of non-innervated human myotubes. Electrical membrane properties of human myotubes were recorded using the whole-cell patch-clamp technique. Cell incubation with recombinant chick neural agrin (1 nM) led to amore negative membrane resting potential. Addition of strophanthidin, a blocker of the Na(+)žK(+) ATPase, depolarized agrin-treated myotubes stronger than control, indicating, in the presence of agrin, a higher contribution of the Na(+)žK(+) ATPase in establishing the resting membrane potential. Indeed, larger amounts of both the alpha1 and the alpha2 isoforms of the Na(+)žK(+) ATPase protein were expressed in agrin-treated cells. A slight but significantdown-regulation of functional apamin-sensitive K(+) channels was observed after agrin treatment. These results indicate that neural agrin mightact as a trophic factor promoting the maturation of membrane electrical properties during differentiation, confirming the role of agrin as a general promoter of muscle development.
Keywords:agrin, myogenesis, electrical membrane properties, skeletal muscle, Na+/K+ pump
Year of publishing:2009
Number of pages:str. 123-131
Numbering:Letn. 29, št. 1
ISSN:0272-4340
UDC:612.74
COBISS_ID:1505235 Link is opened in a new window
Views:1613
Downloads:77
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