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Title:Genetska opredelitev za meticilin občutljivih in proti meticilinu odpornih sevov Staphylococcus aureus, izoliranih iz hemokultur v slovenskih bolnišnicah, s tipizacijo SPA
Authors:ID Špik, Vesna (Author)
ID Grmek-Košnik, Irena (Author)
ID Lorenčič Robnik, Slavica (Author)
ID Žohar Čretnik, Tjaša (Author)
ID Sarjanović, Ljudmila (Author)
ID Kavčič, Martina (Author)
ID Harlander, Dušan (Author)
ID Štrumbelj, Iztok (Author)
ID Tomič, Viktorija (Author)
ID Piltaver-Vajdec, Irena (Author)
ID Fišer, Jerneja (Author)
ID Kolman, Jana (Author)
ID Müller-Premru, Manica (Author)
Files:URL http://www.ivz.si/
 
Language:Slovenian
Work type:Not categorized
Typology:1.02 - Review Article
Organization:UPR - University of Primorska
Abstract:Staphylococcus aureus je drugi najpogostejši bakterijski povzročitelj invazivnih okužb v Sloveniji. S tipizacijo izolatov za meticilin občutljivih S. aureus (MSSA) in proti meticilinu odpornih S. aureus (MRSA) iz hemokultur bolnikov z bakteriemijo iz vse Slovenije v 6- mesečnem obdobju smo poskušali ugotoviti, katerim spa tipom pripadajo invazivni izolati MSSA in MRSA v Sloveniji, kakšna je genetska raznolikost MSSA in MRSA ter preveriti uporabnost tipizacije spa za epidemiološke raziskave S. aureus v Sloveniji. Od 1. septembra 2006 do 28. februarja 2007 smo v 11 slovenskih laboratorijih, ki izvajajo mikrobiološko diagnostiko za 15 bolnišnic, zbirali zaporedno izolirane primoizolate MSSA (največ pet) in zaporedno izolirane primoizolate MRSA (največ pet) iz hemokultur bolnikov. Izolate S. aureus smo tipizirali s tipizacijo spa, to je z določanjem nukleotidnega zaporedja polimorfnega X dela gena spa, ki kodira protein A. Od 58 zbranih izolatov S. aureus iz vse Slovenije smo uspeli tipizirati 54 izolatov. Prevladujoči spa tipi med izolatiMSSA so bili t091, t015 in t005, med izolati MRSA pa t041. Ugotovili smo, da spa tipi, ki prevladujejo med invazivnimi izolati S. aureus pri nas, prevladujejo tudi v sosednjih državah. Tipizacija S. aureus z metodo spa je pokazala veliko genetsko raznolikost invazivnih sevov MSSA in manjšo raznolikost invazivnih sevov MRSA. Tipizacija spa je uporabna tipizacijska metoda za lokalno epidemiologijo MSSA v kratkem in daljšem časovnem obdobju.
Keywords:Staphylococcus aureus, bakteriemija, hemokulture, molekularna tipizacija, tipizacija spa
Year of publishing:2009
Number of pages:str. 78-84
Numbering:Letn. 48, št. 2
PID:20.500.12556/RUP-239 This link opens in a new window
ISSN:0351-0026
UDC:616.9
COBISS.SI-ID:1024084052 This link opens in a new window
Publication date in RUP:15.10.2013
Views:5638
Downloads:53
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Secondary language

Language:English
Abstract:Staphylococcus aureus is the second leading cause of invasive bacterial infections in Slovenia. The aims of this 6-month study were to identify the most prevalent spa types among invasive isolates of methicillin-susceptible S.aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in Slovene hospitals, to determine genetic diversity of MSSA and MRSA, and to test the applicability of spa typing to epidemiological studies of S. aureus in Slovenia. Over a period of six months(1 September 2006 - 28 February 2007), upto five successive methicillin-susceptible (MSSA) blood culture isolates anda maximum of five successive methicillin- resistant (MRSA) blood culture isolates per hospital were obtained from different patients with bacteremia. The isolates were characterized by spa typing based on sequencing of polymorphic region X of the S. aureus protein A gene. Among the 58 isolates collected 54 were succesfully spa typed. The predominant spa types among MSSA isolates were t091, t015, t005, and among MRSA, t041 . The predominant spa types among invasive S. aureus strains isolated in Slovenia predominated also in the neighbouring countries. The study showed high genetic diversity of invasive MSSA isolates and lower genetic diversity of invasive MRSA isolates in Slovenia. Spa typing is a good typing method for short- and long-term epidemiological studies of MSSA on a local scale.
Keywords:Staphylococcus aureus, bacteriemia, blood cultures, molecular typing, spa typing


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