Lupa

Show document

A- | A+ | Print
Title:Effects of inactivity on human muscle glutathione synthesis by a double-tracer and single-biopsy approach
Authors:Agostini, Francesco (Author)
Dalla Libera, Luciano (Author)
Rittweger, Joern (Author)
Mazzucco, Sara (Author)
Jurdana, Mihaela (Author)
Mekjavić, Igor B. (Author)
Pišot, Rado (Author)
Gorza, Luisa (Author)
Narici, Marco (Author)
Biolo, Gianni (Author)
Files:URL http://onlinelibrary.wiley.com/doi/10.1113/jphysiol.2010.198283/pdf
 
Language:English
Work type:Not categorized
Tipology:1.01 - Original Scientific Article
Organization:UPR - University of Primorska
Abstract:Oxidative stress is often associated to inactivity-mediated skeletal muscle atrophy. Glutathione is one of the major antioxidant system stimulated, both at muscular and systemic level, by activation of oxidative processes. We measured changes in glutathione availability, oxidative stress induction and atrophy extent mediated by 35-days of experimental bed rest in vastus lateralis muscle of healthy human volunteers. To assess muscle glutathione synthesis, we applied a novel single-biopsy and double-tracer ([2H2]glycine and [15N]glycine) approach based on evaluation of steady-state precursor incorporation in product. The correlations between the traditional (multiple-samples, one-tracer) and new (one-sample, double-tracer infusion) methods were analyzed in erythrocytes by Passing & Bablok and Altman-Bland tests. Muscle glutathione absolute synthesis rate increased following bed rest from 5.5 1.1 to 11.0 1.5 mmol (kg wet tissue / day)-1 (n=9; p=0.02) while glutathione concentration failed to change significantly. Bed rest induced vastus lateralis muscle atrophy, as assessed by pennation angle changes measured by ultrasonography (from 18.6 1.0 to 15.3 0.9 degrees; p=0.01) and thickness changes (from 2.3 0.2 to 1.9 0.1 cm; p<0.001). Moreover, bed rest increased protein oxidative stress, as measured by muscle protein carbonylation changes (from 0.6 0.1 to 1.00 0.1 Oxy RP-1; p<0.04). In conclusion, we developed in erythrocytes a new minimally-invasive method to determine peptide synthesis rate in human tissues. Application of the new method to skeletal muscle suggests that disuse atrophy is associated to oxidative stress induction as well as to compensatory activation of the glutathione system
Keywords:Antioxidant system, Muscle damage, Oxidative stress, bed rest, muscle glutathione, stable isotopes
Year of publishing:2010
Number of pages:str. 5089-5104
Numbering:Vol. 588, iss. 24
ISSN:0022-3751
UDC:612.7
COBISS_ID:1895635 Link is opened in a new window
Views:2081
Downloads:80
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Categories:Document is not linked to any category.
:
  
Average score:(0 votes)
Your score:Voting is allowed only to logged in users.
Share:Bookmark and Share

Hover the mouse pointer over a document title to show the abstract or click on the title to get all document metadata.

Comments

Leave comment

You have to log in to leave a comment.

Comments (0)
0 - 0 / 0
 
There are no comments!

Back
Logos of partners University of Maribor University of Ljubljana University of Primorska University of Nova Gorica