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Naslov:Effect of ionizing radiation on human skeletal muscle precursor cells
Avtorji:Marš, Tomaž (Avtor)
Čemažar, Maja (Avtor)
Jurdana, Mihaela (Avtor)
Pegan, Katarina (Avtor)
Datoteke:URL http://www.degruyter.com/view/j/raon.2013.47.issue-4/raon-2013-0058/raon-2013-0058.xml?format=INT
 
Jezik:Angleški jezik
Vrsta gradiva:Delo ni kategorizirano
Tipologija:1.08 - Objavljeni znanstveni prispevek na konferenci
Organizacija:FVZ - Fakulteta za vede o zdravju
Opis:Background. Long term effects of different doses of ionizing radiation on human skeletal muscle myoblast proliferation, cytokine signalling and stress response capacity were studied in primary cell cultures.Materials and methods. Human skeletal muscle myoblasts obtained from muscle biopsies were cultured and irradiated with a Darpac 2000 X-ray unit at doses of 4, 6 and 8 Gy. Acute effects of radiation were studied by interleukin - 6 (IL-6) release and stress response detected by the heat shock protein (HSP) level, while long term effects were followed by proliferation capacity and cell death.Results. Compared with non-irradiated control and cells treated with inhibitor of cell proliferation Ara C, myoblast proliferation decreased 72 h post-irradiation, this effect was more pronounced with increasing doses. Post-irradiation myoblast survival determined by measurement of released LDH enzyme activity revealed increased activity after exposure to irradiation. The acute response of myoblasts to lower doses of irradiation (4 and 6 Gy) was decreased secretion of constitutive IL-6. Higher doses of irradiation triggered a stress response in myoblasts, determined by increased levels of stress markers (HSPs 27 and 70).Conclusions. Our results show that myoblasts are sensitive to irradiation in terms of their proliferation capacity and capacity to secret IL-6. Since myoblast proliferation and differentiation are a key stage in muscle regeneration, this effect of irradiation needs to be taken in account, particularly in certain clinical conditions.
Ključne besede:myoblasts, irradiation, proliferation, interleukin 6, muscle regeneration, apoptosis
Leto izida:2013
Založnik:Croatian Medical Association - Croatian Society of Radiology
Št. strani:str. 376-381, V
Številčenje:Vol. 47, no. 4
ISSN:1318-2099
UDK:612:551.521
COBISS_ID:512423481 Povezava se odpre v novem oknu
DOI:10.2478/raon-2013-0058 Povezava se odpre v novem oknu
Število ogledov:1882
Število prenosov:104
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
Področja:Gradivo ni uvrščeno v področja.
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Skupna ocena:(0 glasov)
Vaša ocena:Ocenjevanje je dovoljeno samo prijavljenim uporabnikom.
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