In silico analysis of phages-nanogen interplayMalat, Ihab (Avtor) Drancourt, Michel (Avtor) Henrissat, B. (Avtor) Grine, Ghiles (Avtor) Archaeal viromeMethanogensCRISPR-SpacersCaudoviricetesHuman microbiomeMicrobial diversityMobile genetic elementsNanoarchaeaNanogenMethanobrevibacter smithiiMethanobrevibacter oralisDigestive tract nanoarchaea Nanopusillus massiliensis and Candidatus Nanopusillus phoceensis form symbiotic nanogens with residing methanogens Methanobrevibacter oralis and Methanobrevibacter smithii, preventing their translocation by unknown mechanisms. We found that 0.45% of M. oralis YH nanogen genome encoding 18 proteins and 1.12% of M. smithii KB11 nanogen genome encoding 32 proteins were of Caudoviricetes and NAV1 origin. In block encoded glycosyltransferase and S-adenosyl-methionine-dependent methyltransferase confer nanogens (but not methanogens) a capacity in cell wall glycosylation. This hypothetical deduced cell wall glycosylation could prevent pseudomurein endo-isopeptidase-mediated interactions of nanogens with digestive tract virome and further nanogen translocation. These observations renew understanding of methanogen and nanogen-associated physiology and pathologies in the digestive tract and beyond.20262026-06-18 10:00:52Članek v reviji23156sl