20.500.12556/RUP-21532 The impact of Aureobasidium melanogenum cells and extracellular vesicles on human cell lines Aureobasidium melanogenum is a black yeast-like fungus that occurs frequently both in nature and in domestic environments. It is becoming increasingly important as an opportunistic pathogen. Nevertheless, its effect on human cells has not yet been studied. In this study, we investigated the effect of A. melanogenum cells and extracellular vesicles (EVs) on human cell lines A549 (human lung cells), HDFa (human dermal fibroblasts), and SH-SY5Y (human neuroblastoma cells). Scanning electron microscopy (SEM) showed no direct interaction between A. melanogenum cells and human cell lines, but there were some changes in HDFa cells. As a possible cause for this change, we tested the cytotoxic effect of EVs from A. melanogenum on the same cell lines. We isolated EVs from the fungus and prepared three different pools: a non-melanin pool (containing mainly EVs), a melanin pool (containing mainly melanin nanoparticles), and a total pool (containing both EVs and melanin nanoparticles). All three pools were characterized and then added to human cell lines to test their cytotoxicity. Unlike in some other fungal opportunistic pathogens, no effects of fungal EVs on human cell viability were observed. Therefore, the opportunistic potential of A. melanogenum remains only partially understood. extracellular vesicles Aureobasidium melanogenum pathogenesis melanin human cell line zunajcelični vezikli Aureobasidium melanogenum patogeneza melanin človeška celična linija true false false Angleški jezik Slovenski jezik Članek v reviji 2025-08-07 10:55:58 2025-08-07 10:56:00 2025-08-08 03:03:36 0000-00-00 00:00:00 2025 0 0 str. 1-11 iss. 1 , ǂarticle no. ǂ1413 Vol. 15 2025 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.61:616-092 2045-2322 10.1038/s41598-024-84189-3 221745411 RAZ_Cernosa_Anja_2025.pdf RAZ_Cernosa_Anja_2025.pdf 1 82623083004FF0820028BC0C05638AFB ee6e14968b354df93d8a396113a0dfb1796860fde365e6c64eae7dd255c5838e 792f7d72-736c-11f0-8f0b-005056ac49c0 https://repozitorij.upr.si/Dokument.php?lang=slv&id=31314 https://www.nature.com/articles/s41598-024-84189-3 1 5d32ee84-736c-11f0-8f0b-005056ac49c0 https://repozitorij.upr.si/Dokument.php?lang=slv&id=31313 Univerza na Primorskem 0 0 0