20.500.12556/RUP-22216 First EST-SSRs of Helichrysum italicum (Roth) G. Don (Asteraceae) revealed insights into the genetic diversity and population structure in Corsica Helichrysum italicum (Roth) G. Don (Asteraceae) is a valuable medicinal and aromatic plant native to a variety of habitats across the Mediterranean region. However, genetic studies of this morphologically diverse species have been limited by the scarcity of species-specific DNA markers. To address this limitation, we generated the first de novo transcriptome assembly comprising 24,806 transcripts from young shoots containing leaves and flowers, developed EST-SSR markers, and evaluated their utility in population genetic analysis. Seventy-eight primer pairs were designed, of which 23 showed successful amplification, polymorphism, and transferability to Helichrysum litoreum Guss. and Helichrysum arenarium (L.) Moench. A subset of 12 EST-SSRs was used to genotype 270 individuals from 12 natural populations of H. italicum in Corsica (France), along with one outgroup population from Croatia. The polymorphic information content ranged from 0.250 to 0.796, and Shannon’s information index ranged from 0.588 to 1.843, indicating the markers’ suitability for popu- lation genetic studies. Analysis of molecular variance revealed that 15% of the total genetic variation was attributable to differences among populations. Discriminant analysis of principal components and Bayesian clustering in STRUCTURE identified distinct popu- lation clusters corresponding to geographic locations. Notably, the southernmost coastal populations were clearly differentiated from the others. Helichrysum italicum immortelle de novo transcriptome genic microsatellite markers EST-SSRs population study Corsica Mediterranean Basin Helichrysum italicum laški smilj de novo transkriptom genski mikrosatelitski označevalci EST-SSRs populacijska študija Korzika Sredozemski bazen true false true Angleški jezik Slovenski jezik Članek v reviji 2025-12-17 09:10:33 2025-12-17 09:10:36 2025-12-20 03:04:20 0000-00-00 00:00:00 2025 0 0 str. 1-31 iss. 24, [article no.] 3794 Vol. 14 Dec.-2 2025 0000-00-00 Zaloznikova NiDoloceno apc 0000-00-00 0000-00-00 2025-12-12 575.113.2 2223-7747 10.3390/plants14243794 261785091 RAZ_Gabrovsek_Petra_2025.pdf RAZ_Gabrovsek_Petra_2025.pdf 1 3C7827CD66115508A8EB647DD4ABECE5 90bea03c783f6d12764e2fcfc415ece6e41d3ed4d01c8ea5a666dfac6eb08407 521ce4d7-db20-11f0-8f0b-005056ac49c0 https://repozitorij.upr.si/Dokument.php?lang=slv&id=32257 https://www.mdpi.com/2223-7747/14/24/3794 1 2decce4d-db20-11f0-8f0b-005056ac49c0 https://repozitorij.upr.si/Dokument.php?lang=slv&id=32256 Fakulteta za matematiko, naravoslovje in informacijske tehnologije Fakulteta za vede o zdravju 0 0 0