1. Effective cryopreservation of post mortem-collected roe deer gametes by evaluation of post-thaw oocyte and sperm characteristics and in vitro fertilizationAnna Justyna Korzekwa, Elena Bužan, Boštjan Pokorny, Gulsum Ummu Boztepe, Marek Lecewicz, Władysław Kordan, 2025, original scientific article Abstract: The aim was to evaluate the effectiveness of semen cryopreservation and oocyte vitrification in roe deer as a potential method of gamete preservation for endangered deer species. Sperm were isolated from the cauda epididymis of fourteen bucks (n = 14). The motility measure (CASA) and morphology of fresh semen (FS) and frozen–thawed semen (TS) were compared. A hyaluronic binding assay was used to distinguish between mature FS spermatozoa expressing hyaluronan receptors and immature FS lacking these receptors, and the mitochondrial membrane potential (MMP) in TS was determined (flow cytometry). A Sperm–Hyaluronan Binding Assay (HBA) showed a viability rate of 61.9% in FS and 78.2% in TS. Oocytes received from eight does (n = 8) underwent a viability test and vitrification, and fresh oocytes from the other eight does (n = 8) were fertilized with TS and embryos were cultured until the blastocyst stage. The number of isolated oocytes, cumulus–oocyte complexes (COCs), cleaved embryos, and expanded blastocysts was evaluated. Higher percentages of morphological factors (acrosome, head, midpiece, and tail shape) were observed in FS compared to TS, whereas the motility and progressive movement were greater in TS (p ≤ 0.001). The viability was 50.5% and MMP was 40.6% in TS. A total of 311 oocytes were collected and from 150 COCs and 125 blastocysts developed. The viability of thawed oocytes after vitrification was 81%. The viability of vitrified oocytes and cryopreserved sperm confirmed the effectiveness of freezing protocols and highlights the potential for their implementation in other deer species.
Keywords: roe deer, semen, oocytes, fertilization
Published in RUP: 13.10.2025; Views: 307; Downloads: 12
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2. The role of community science in DNA-based biodiversity monitoringCarolina Corrales, Karolina Bacela-Spychalska, Elena Bužan, Torbjørn Ekrem, Sónia Ferreira, William Goodall-Copestake, Elaine van Ommen Kloeke, Peter M. Hollingsworth, Sarah J. Bourlat, 2025, original scientific article Abstract: The mutual interest in nature by the general public and scientists has led to many collaborations, past and present. Community science shows great potential for monitoring species occurrences and distributions, especially in combination with scalable and (semi)-automated methods such as DNA-based monitoring, helping to obtain data from a broader geographic and temporal range than would be possible by the scientific community alone. Here, we present an overview of the complementarity between community science and DNA-based biomonitoring through examples from ongoing projects. The involvement of hobby experts is particularly crucial for building up the necessary species reference databases that enable DNA-based monitoring. Based on this overview, we identify some key points related to learning opportunities and participant recognition to maximise the success, impact and benefit of community participants in DNA-based monitoring.
Keywords: eDNA, community science, genetics
Published in RUP: 13.10.2025; Views: 259; Downloads: 2
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3. Piškurji Slovenije : pregled in primerjava metod izlovaLuka Ložar, 2025, undergraduate thesis Keywords: elektroribolov, Eudontomyzon vladykovi, Lampetra zanandreai, metoda ZZRS, monitoring, piškurji, posplošeni linearni mešani model, učinkovitost izlova, vzorčenje, diplomske naloge
Published in RUP: 07.10.2025; Views: 373; Downloads: 0 |
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5. Navigating COP16’s digital sequence information outcomes : what researchers need to do in practiceMelania Muñoz-García, Amber Hartman Scholz, 2025, other scientific articles Abstract: The UN Convention on Biological Diversity adopted new rules for sharing benefits from publicly available genetic sequence data, also known as digital sequence information (DSI). In this Opinion, the authors describe the key elements researchers need to be aware of, address real-life questions, and explain the practical implications of these rules for research and development.
Keywords: COP16, digital sequence information, sequences, research
Published in RUP: 26.09.2025; Views: 408; Downloads: 6
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6. Ocena invazivnega potenciala tujerodnih sesalcev v Sloveniji z uporabo orodja TAS-ISK : zaključna nalogaAnja Nabergoj, 2025, undergraduate thesis Keywords: tujerodni sesalci, invazivne vrste, invazivni potencial, ocena tveganja, TAS-ISK, Slovenija, podnebne spremembe, biotska raznovrstnost, zaključne naloge
Published in RUP: 06.09.2025; Views: 447; Downloads: 13
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7. Red deer resequencing reveals the importance of sex chromosomes for reconstructing Late Quaternary eventsMenno J. de Jong, Gabriel Anaya, Aidin Niamir, Javier Pérez-González, Camilla Broggini, Alberto Membrillo del Pozo, Marcel Nebenfuehr, Eva de la Peña, Jordi Ruiz-Olmo, Jose Manuel Seoane, Elena Bužan, Laura Iacolina, 2025, original scientific article Abstract: Sex chromosomes differ in their inheritance properties from autosomes, and hence may encode complementary information about past demographic events. We compiled and analysed a range-wide resequencing dataset of the red deer (Cervus elaphus), one of the few Eurasian herbivores of the Late Pleistocene megafauna still found throughout much of its historic range. Our analyses of 144 whole genomes reveal striking discrepancies between the population clusters suggested by autosomal and X-chromosomal data. We postulate that the genetic legacy of Late Glacial population structure is better captured and preserved by the X chromosome than by autosomes, for two reasons. First, X chromosomes have a lower Ne and hence lose genetic variation faster during isolation in glacial refugia, causing increased population differentiation. Second, following postglacial recolonisation and secondary contact, immigrant males pass on their X chromosomes to female offspring only, which effectively halves the migration rate when gene flow is male-mediated. Our study illustrates how a comparison between autosomal and sex chromosomal phylogeographic signals unravels past demographic processes which otherwise would remain hidden.
Keywords: red deer, sex chromosome, genomics
Published in RUP: 08.08.2025; Views: 421; Downloads: 5
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8. Od genov do vedenja in naprejSimona Kralj-Fišer, 2024, reviewed university, higher education or higher vocational education textbook Keywords: vedenjska genetika, dedovanje, stres, stresni odziv, agresivnost, navezanost, osebnost, spol, duševne motnje, čustvene motnje, anksioznost, shizofrenija
Published in RUP: 10.04.2025; Views: 859; Downloads: 50
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