1. Processing and mounting phlebotomine sand flies : a consensus guidelineFano José Randrianambinintsoa, Laure Augendre, Jorian Prudhomme, Jean-Philippe Martinet, Mathieu Loyer, Nalia Mekarnia, Hocine Kerkoub, Farzana Khan Perveen, Antoine Huguenin, Vladimir Ivović, 2026, review article Abstract: This article provides a comprehensive guide for the processing and mounting of phlebotomine sand fly specimens, which is crucial for species identification and pathogen detection and isolation. It discusses a range of techniques suitable for both field and laboratory settings. The guide includes detailed instructions on sand fly collec- tion, handling, covering, and euthanasia (recommending dry freezing or CO2 over chemicals) as well as conservation strategies, such as cold storage and preservation in ethanol. The quality of preparation of certain anatomical structures (genital organs, head and wings) is essential for their proper microscopic observation and is described in this work. The article also presents detailed sample processing, including the clearing process with agents such as potassium hydrox- ide then Marc-André solution. The mounting process compares different media, emphasizing their optical properties and preservation potential. Hoyer fluid (also known as chloral gum) is recommended for quick observation, particularly for spermathecae, due to its clarity, although it is not suitable for long-term storage. Other media discussed include polyvinyl alcohol, EuparalÒ (for limited water tolerance), and Canada balsam (a hydrocarbon-soluble medium), with the latter two offering long-term preservation capabilities. Innovative molecular biology approaches such as DNA sequencing and MALDI-ToF, which require particular attention to sample processing, are also addressed. Furthermore, short video clips illustrating various mounting techniques as well as translations in many different languages are provided, allowing the guideline to reach the diverse needs and expectations of the global scientific community.
Keywords: mounting, Phlebotomine sand fly, Hoyer fluid, Marc-André solution, Chloral gum, Polyvinyl alcohol, Euparal®, Canada balsam, Leishmania isolation, field conditions, culture, dissection, molecular biology, MALDI-ToF, type-specimens
Published in RUP: 03.04.2026; Views: 152; Downloads: 6
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2. Data from the Researcher Mental Health Observatory STAIRCASE surveyJana Lasser, Stefan T. Mol, Alja Čontala, Ana Slavec, Andreja Zulim de Swarte, Anna Khachatryan, Anna Maria Eleuteri, Anupoma Haque, Baiba Jansone, Blerina Vrenozi, Mateja Erce Paoli, 2025, other scientific articles Abstract: The data presented here derives from the STAIRCASE survey on researcher mental health. The survey reached 4,296 researchers predominantly from European countries who completed an online questionnaire about mental health outcomes such as depression, anxiety, stress, burnout, and well-being, as well as working conditions and leadership behaviour. Data and materials of the study are available at https://doi.org/10.21249/DZHW:remo:1.0.0 as a fully anonymised downloadable Campus Use File and a pseudonymised Scientific Use File, accessible in a secure remote analysis environment. Data can be reused for secondary analyses, educational purposes, or combined with similar data sets.
Keywords: mental health, occupational health, researchers, working conditions
Published in RUP: 19.12.2025; Views: 473; Downloads: 9
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4. THE RELIGIOUS AND MENTAL HEALTH DETERMINANTS OF THE COVID-19’S FEAR IN THE CEE COUNTRIESSerban Olah, Katarzyna Bałandynowicz-Panfil, Janez Štebe, Mojca Šoštarič Zvonar, Ana Slavec, Adrienn Kaszás, Győző Pék, Małgorzata Łosiewicz, Magdalena Iwanowska, 2024, original scientific article Abstract: The research analyses the relationship between fear and religiosity, particularly focusing on religious habits and intrinsic religiosity in the context of the covid-19 pandemic. A total number of 2212 questionnaires have been collected in Romania, Poland, Slovenia and Hungary in 2021 on the non-vaccinated population. The questionnaire covers topics such as vaccination, religiosity and socio-demographics, and includes the DASS 21 psychological scale, which measures depression, anxiety and stress symptoms. Furthermore, the questionnaire incorporated a specific question regarding the fear of SARS-CoV-2 virus. The findings show that the intrinsic religiosity, age, stress, anxiety and exposure to the news from the traditional media or to the information coming from professional sources seem to increase the degree of fear during the pandemic context. The study yields significant implications for understanding the complex interplay of factors shaping the vaccination attitudes of this group, as well as how they obtain information. This may contribute to developing more effective communication channels for people with passive strategies for coping with fear and anxiety. The need to seek information in times of uncertainty and attitudes that indicate a higher level of intimate religiosity can be leveraged to build more effective coping strategies for unexpected external events or situations that cause fear and anxiety. This is important in light of the ongoing development of Central and Eastern European countries and their specific social and economic background.
Keywords: religiosity, pandemic, DASS 21, socio-economic, conditions
Published in RUP: 23.04.2024; Views: 4555; Downloads: 57
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