1. The impact of Aureobasidium melanogenum cells and extracellular vesicles on human cell linesAnja Černoša, Cene Gostinčar, Marija Holcar, Rok Kostanjšek, Metka Lenassi, Nina Gunde-Cimerman, 2025, original scientific article Abstract: Aureobasidium melanogenum is a black yeast-like fungus that occurs frequently both in nature and in domestic environments. It is becoming increasingly important as an opportunistic pathogen. Nevertheless, its effect on human cells has not yet been studied. In this study, we investigated the effect of A. melanogenum cells and extracellular vesicles (EVs) on human cell lines A549 (human lung cells), HDFa (human dermal fibroblasts), and SH-SY5Y (human neuroblastoma cells). Scanning electron microscopy (SEM) showed no direct interaction between A. melanogenum cells and human cell lines, but there were some changes in HDFa cells. As a possible cause for this change, we tested the cytotoxic effect of EVs from A. melanogenum on the same cell lines. We isolated EVs from the fungus and prepared three different pools: a non-melanin pool (containing mainly EVs), a melanin pool (containing mainly melanin nanoparticles), and a total pool (containing both EVs and melanin nanoparticles). All three pools were characterized and then added to human cell lines to test their cytotoxicity. Unlike in some other fungal opportunistic pathogens, no effects of fungal EVs on human cell viability were observed. Therefore, the opportunistic potential of A. melanogenum remains only partially understood.
Keywords: extracellular vesicles, Aureobasidium melanogenum, pathogenesis, melanin, human cell line
Published in RUP: 07.08.2025; Views: 348; Downloads: 3
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3. Point-ellipse configurations and related topicsGábor Gévay, Nino Bašić, Jurij Kovič, Tomaž Pisanski, 2021, original scientific article Keywords: point-line configuration, conic section, point-ellipse configuration, point-conic configuration, Levi graph, Carnot's theorem
Published in RUP: 18.10.2021; Views: 3921; Downloads: 27
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6. In-line measurement of wood surface roughnessJakub Michal Sandak, Kazimierz A. Orłowski, Anna Malgorzata Sandak, Daniel Chuchała, Piotr Taube, 2019, published scientific conference contribution abstract Keywords: wood surface roughness, triangulation scanner, surface defects, on-line, at-line
Published in RUP: 11.02.2020; Views: 2783; Downloads: 109
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8. In situ assessment of structural timber using semi-destructive techniquesT. Tannert, Ronald W. Anthony, Bohumil Kasal, Michal Kloiber, Maurizio Piazza, Mariapaola Riggio, Frank Rinn, Robert Widmann, Nobuyoshi Yamaguchi, 2014, original scientific article Keywords: resistance drilling, core drilling, glue line test, tension micro-specimens, screw withdrawal, needle penetration, pin pushing, hardness test
Published in RUP: 19.11.2018; Views: 3396; Downloads: 184
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