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5. First EST-SSRs of Helichrysum italicum (Roth) G. Don (Asteraceae) revealed insights into the genetic diversity and population structure in CorsicaPetra Gabrovšek, Matjaž Hladnik, Dunja Bandelj, Zala Jenko Pražnikar, Saša Kenig, Félix Tomi, Marc Gibernau, Slavko Brana, Alenka Baruca Arbeiter, 2025, izvirni znanstveni članek Opis: Helichrysum italicum (Roth) G. Don (Asteraceae) is a valuable medicinal and aromatic plant native to a variety of habitats across the Mediterranean region. However, genetic studies of this morphologically diverse species have been limited by the scarcity of species-specific DNA markers. To address this limitation, we generated the first de novo transcriptome assembly comprising 24,806 transcripts from young shoots containing leaves and flowers, developed EST-SSR markers, and evaluated their utility in population genetic analysis. Seventy-eight primer pairs were designed, of which 23 showed successful amplification, polymorphism, and transferability to Helichrysum litoreum Guss. and Helichrysum arenarium (L.) Moench. A subset of 12 EST-SSRs was used to genotype 270 individuals from 12 natural populations of H. italicum in Corsica (France), along with one outgroup population from Croatia. The polymorphic information content ranged from 0.250 to 0.796, and Shannon’s information index ranged from 0.588 to 1.843, indicating the markers’ suitability for popu- lation genetic studies. Analysis of molecular variance revealed that 15% of the total genetic variation was attributable to differences among populations. Discriminant analysis of principal components and Bayesian clustering in STRUCTURE identified distinct popu- lation clusters corresponding to geographic locations. Notably, the southernmost coastal populations were clearly differentiated from the others.
Ključne besede: Helichrysum italicum, immortelle, de novo transcriptome, genic microsatellite markers, EST-SSRs, population study, Corsica, Mediterranean Basin
Objavljeno v RUP: 17.12.2025; Ogledov: 171; Prenosov: 6
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6. Polyphenolic composition, genetic profile and in vitro antioxidant and cytotoxic activities of artichoke varieties “Strunjanska articoka”, “Romanesco” and "Violetto di Romagna"Katja Kramberger, Alenka Baruca Arbeiter, Ana Petelin, Petra Gabrovšek, Nemanja Teslić, Saša Kenig, Aleksandra Mišan, Dunja Bandelj, Alena Stupar, Zala Jenko Pražnikar, 2025, izvirni znanstveni članek Opis: In recent years, artichokes have gained attention as functional foods due to their polyphenol content and health benefits. While Italian varieties have been widely studied, the Slovenian landrace “Strunjanska articoka” remains largely unexplored. This study compared “Strunjanska articoka” with two Italian varieties, “Romanesco” and “Violetto di Romagna”, assessing genetic profiles, chemical composition, antioxidant activity, and effects on lipid accumulation. The genetic analysis confirmed the uniqueness and clear differentiation of the landrace “Strunjanska articoka” from the two commercial varieties. Analysing the profiles of phenolic compounds in the leaf extracts of these morphologically different artichoke varieties underlines their unique phytochemical composition, which translates into different health benefits. “Romanesco” showed the highest free radical scavenging activity (EC50 = 100 µg/mL), followed by “Strunjanska articoka” (EC50 = 143 µg/mL), and “Violetto di Romagna” (EC50 = 160 µg/mL). Importantly, “Strunjanska articoka” demonstrated superior cytoprotective effects against oxidative stress in HepG2 and CCD112CoN cells. It also reduced lipid accumulation in HepG2 cells, along with “Violetto di Romagna,” whereas “Romanesco” did not. These findings justify further investigation into the potential therapeutic effects of “Strunjanska articoka” and support the rationale for its cultivation.
Ključne besede: artichoke leaf extracts, phenolic compounds, antioxidative potential, lipid accumulation
Objavljeno v RUP: 06.12.2025; Ogledov: 258; Prenosov: 4
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7. Genetic diversity of cultivated figs (Ficus carica L.) from the Eastern Adriatic Coast screened by SSR markersMira Radunić, Alenka Baruca Arbeiter, Mate Čarija, Katarina Hančević, Danijela Poljuha, Miroslav Čizmović, Frane Strikić, Dunja Bandelj, 2025, izvirni znanstveni članek Opis: Fig tree is fruit species, widely distributed throughout the Adriatic region, and is valued as a key component of the Mediterranean diet. Despite its importance for the region’s fruit-growing industry, the genetic makeup of Adriatic fig cultivars has not yet been thoroughly investigated. A comprehensive molecular characterization of 213 fig accessions from the Eastern Adriatic Coast was conducted to assess genotype-specific molecular profiles and genetic diversity using seven Simple Sequence Repeat markers. Aim of this research was to catalogue the cultivars and clarify cases of synonymy and homonymy. The research included three genebank collections: the Institute of Adriatic Crops in Split (Croatia), the Institute of Agriculture and Tourism in Poreč (Croatia), and the Centre for Subtropical Cultures Bar, Biotechnical Faculty University of Montenegro, alongside additional accessions collected from family farms and nursery in Croatia and Slovenia. The analysis revealed 122 alleles, indicating substantial genetic diversity. A total of 80 unique genotypes were identified, along with 51 cases of synonymy or homonymy and 24 accessions with unknown nomenclature. The phylogenetic tree highlighted the heterogeneous nature of the fig population along the East Adriatic Coast. Notably, each of the three major clusters contained accessions from all three countries, underscoring the high genetic diversity across the region. These defined molecular profiles provide a foundation for the effective conservation of fig cultivars, the establishment of high-quality mother blocks, nursery production, and future breeding programs.
Ključne besede: plant genetic resources, fig germplasm, genetic profiles, SSR markers, synonyms and homonyms
Objavljeno v RUP: 11.06.2025; Ogledov: 847; Prenosov: 33
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9. Možnosti nadaljnjega razvoja vinskega in gastronomskega turizma na kmetijah : zaključno poročiloSimon Kerma, Aleš Gačnik, Igor Jurinčič, Emil Juvan, Štefan Bojnec, Dunja Bandelj, Alenka Baruca Arbeiter, Matjaž Hladnik, Barbara Lampič, Dejan Cigale, Irma Potočnik Slavič, Martina Robačer, Franc Bavec, Martina Bavec, 2023, končno poročilo o rezultatih raziskav Ključne besede: agrarna geografija, podeželje, turistična geografija, vinski turizem, gastronomski turizem, turizem na kmetijah, razvoj turizma, Slovenija
Objavljeno v RUP: 20.12.2023; Ogledov: 3151; Prenosov: 70
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