1. Intraspecific geographical variability of Phlebotomus perniciosus assessed by MALDI-TOF MS protein profilingVít Dvořák, Carla Maia, Rafael Marmé, José Risueño, Pedro Pérez-Cutillas, Eduardo Berriatua, Julie Sevila, Nalia Mekarnia, Jorian Prudhomme, Fano José Randriananambinintsoa, Vladimir Ivović, Katja Adam, 2026, izvirni znanstveni članek Opis: Background Matrix‑assisted laser desorption/ionization time‑of‑flight mass spectrometry (MALDI‑TOF MS) pro‑ tein profiling has emerged over the last decade as a method of choice for species identification of many medically important arthropods. However, the influence of intraspecific variability on the performance of this popular technique has seldom been tested. This study provides the first standardized comparison of different geographical popula‑ tions of Phlebotomus perniciosus, a vector of Leishmania infantum and Toscana virus in the western Mediterranean, by MALDI‑TOF MS protein profiling. Methods Phlebotomus perniciosus males were collected in five countries (Portugal, Spain, France, Italy, Croa‑ tia) that represent most of its distribution in Europe. All samples were trapped, stored and processed according to a highly standardized protocol to avoid effects other than geographical origin on their protein spectra acquired by MALDI‑TOF MS protein profiling. The obtained protein spectra were compared with laboratory‑reared specimens of Ph. perniciosus. Results Twenty‑two analysed specimens from five geographical populations provided protein spectra that were highly similar, species‑specific and clustering according to their quality. No grouping according to geographical origin was observed, and the protein spectra of field‑collected specimens showed similar composition and complexity to spectra from Ph. perniciosus laboratory colony‑reared in captivity for several decades. Conclusions Our findings demonstrate that in samples of a same sex, with the same collection method and storage time, MALDI‑TOF MS protein profiling does not reflect the geographical origin of analysed specimens, confirming the value of this technique for high‑fidelity and reproducible species identification of sand flies regardless of their geographical origin.
Ključne besede: intraspecific geographical variability, Phlebotomus perniciosus, MALDI-TOF
Objavljeno v RUP: 29.06.2026; Ogledov: 221; Prenosov: 5
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2. Recent advances in Phlebotomine sand fly research : a review based on studies presented at ISOPS XIVladimir Ivović, Gioia Bongiorno, Petr Volf, Yara Traub Cseko, Jeffrey Jon Shaw, Dia Elnaiem, Shaden Kamhawi, Eva Iniguez, Carla Maia, Suzana Blesić, 2025, pregledni znanstveni članek Opis: The 11th International Symposium on Phlebotomine Sand flies (ISOPS XI) took place in Portorož, Slovenia, in September 2024 and brought together experts from around the world to discuss recent advances in the biology, ecology and control of phlebotomine sand flies and the pathogens they transmit. This report summarises the key findings of the symposium and is organised thematically by session. Key topics included the development of refined experimental models of Leishmania transmission, new insights into the interactions between vector, parasite and microbiota, and the detection of Leishmania donovani in new geographic regions. Advances in molecular diagnostics and surveillance technologies were emphasised, as were emerging concerns about insecticide resistance. The potential of paratransgenesis and symbiont-based vector control approaches was also emphasised. In a separate session, the CLIMOS project was presented, which integrates climate monitoring, ecological modelling and public health tools to develop an early warning system (EWS) for sand fly-borne diseases. Overall, the contributions to the symposium reflect the dynamic development of sand fly research in response to global environmental change and emphasise the importance of international collaboration in combating emerging vector-borne diseases.
Ključne besede: Phlebotomine sand flies, vector competence, surveillance, climate change, taxonomy, ISOPS
Objavljeno v RUP: 30.10.2025; Ogledov: 663; Prenosov: 9
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