1. A new wildlife monitoring app in Slovenia : a pilot evaluation of data quality and useŽiga Velkavrh, Luka Duniš, Boštjan Pokorny, Hubert Potočnik, Elena Bužan, 2026, izvirni znanstveni članek Opis: Wildlife monitoring is crucial for successful conservation and management of wildlife populations. To support modern wildlife monitoring approaches in Slovenia, we developed SRNA, a nationwide citizen science appli- cation that collects additional metadata on several terrestrial vertebrate species. The app is designed for two groups of citizen scientists, hunters and other nature observers. To foster learning, it features a photo-based wildlife identification quiz and species identification guides. This pilot study evaluated app use and data quality with two aims: (1) to assess users’ ability to identify wildlife species from photos, focusing on taxa expected to be challenging to identify, and to test whether identification accuracy varies across mammal groups; (2) to analyse wildlife observation data submitted through SRNA. Users were successful in identifying several distinctive species but had some difficulty distinguishing between certain mustelids, and between golden jackal and grey wolf, indicating priorities for targeted educational campaigns. Biology-related educational background facilitated identification of mustelids and, to a lesser extent, canids. In its first year, SRNA collected 1633 observations via 742 reports, predominantly of European roe deer. Hunters and nature observers contributed complementary data: differences were observed not only in the number of reported species and amount of data they provided, but also in reporting patterns across biogeo- graphic regions, reporting rates, and the average delay time between encounters and submissions. This study demonstrates that SRNA provides promising complementary data for wildlife monitoring, but also highlights data quality limitations (biases and verification issues) that must be taken into account when using SRNA data. Ključne besede: application for wildlife monitoring, citizen science, mammals, Slovenia, SRNA app, Wildlife identification quiz Objavljeno v RUP: 08.04.2026; Ogledov: 160; Prenosov: 7
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2. Biodiversity Genomics Europe (BGE) Project – abridged grant proposalDimitris Koureas, Pedro Beja, Mark L. Blaxter, Astrid Böhne, Sarah J. Bourlat, Torbjørn Ekrem, Brent C. Emerson, Katharina F. Heil, José Melo-Ferreira, Ben Price, Elena Bužan, Laura Iacolina, 2026, pregledni znanstveni članek Opis: The Biodiversity Genomics Europe (BGE) Project has the overarching aim of accelerating the use of genomic science to enhance understanding of biodiversity, monitor biodiversity change, and guide interventions to address its decline. The BGE Project comprises activities focused on DNA Barcoding (Barcoding Stream) and Reference Genome Generation (Genomes Stream) for eukaryotic species across Europe, bringing together two European networks: the International Barcode of Life in Europe (iBOL Europe) and the European Reference Genome Atlas (ERGA). This publication is an abridged version of the successful grant proposal developed jointly by iBOL Europe and ERGA in response to the Horizon Europe call HORIZON-CL6-2021-BIODIV-01-01. Two key strands of genomic science form the basis of this proposal: DNA barcoding - sequencing short, standardised genomic regions to tell the world’s species apart, transforming the speed of completion of the inventory of life on Earth and providing the foundations of a global bio-surveillance system for biodiversity; and genome sequencing - generating high-quality complete reference genomes for all species on Earth, transforming understanding of biodiversity at the genetic level, and delivering fundamental knowledge of how biological systems function and how species respond and adapt to environmental change. The BGE Project objectives are focused on (i) Capacity: To establish functioning biodiversity genomics networks at the European level to connect and grow community capacity to use genomic tools to tackle the biodiversity crisis; (ii) Production: To establish and implement large-scale biodiversity genomic data generation pipelines for Europe to accelerate the production and accessibility of genomic data for biodiversity characterisation, conservation, and biomonitoring; and (iii) Application: To apply genomic tools to enhance understanding of pan-European biodiversity and biodiversity declines to improve the efficacy of management interventions and biomonitoring programmes. Ključne besede: biodiversity genomics, DNA barcodes, ERGA, Horizon Europe, iBOL Europe, reference genomes Objavljeno v RUP: 03.03.2026; Ogledov: 242; Prenosov: 17
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3. Dealing with the complexity of effective population size in conservation practiceAncuta Cotovelea Fedorca, Joachim Mergeay, Adejoke O. Akinyele, Tamer Albayrak, Iris Biebach, Alice Brambilla, Pamela Burger, Elena Bužan, Ino Čurik, Roberta Gargiulo, Marjana Westergren, 2024, pregledni znanstveni članek Opis: Effective population size (Ne) is one of the most important parameters in evolutionary biology, as it is linked to the long-term survival capability of species. Therefore, Ne greatly interests conservation geneticists, but it is also very relevant to policymakers, managers, and conservation practitioners. Molecular methods to estimate Ne rely on various assumptions, including no immigration, panmixia, random sampling, absence of spatial genetic structure, and/or mutation-drift equilibrium. Species are, however, often characterized by fragmented populations under changing environmental conditions and anthropogenic pressure. Therefore, the estimation methods' assumptions are seldom addressed and rarely met, possibly leading to biased and inaccurate Ne estimates. To address the challenges associated with estimating Ne for conservation purposes, the COST Action 18134, Genomic Biodiversity Knowledge for Resilient Ecosystems (G-BiKE), organized an international workshop that met in August 2022 in Brașov, Romania. The overarching goal was to operationalize the current knowledge of Ne estimation methods for conservation practitioners and decision-makers. We set out to identify datasets to evaluate the sensitivity of Ne estimation methods to violations of underlying assumptions and to develop data analysis strategies that addressed pressing issues in biodiversity monitoring and conservation. Referring to a comprehensive body of scientific work on Ne, this meeting report is not intended to be exhaustive but rather to present approaches, workshop findings, and a collection of papers that serve as fruits of those efforts. We aimed to provide insights and opportunities to help bridge the gap between scientific research and conservation practice. Ključne besede: Ne, effective population size, inbreeding Objavljeno v RUP: 04.02.2026; Ogledov: 357; Prenosov: 1
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4. From permits to samples : addressing key challenges for high-quality reference genome generation in EuropeKatja Reichel, Jaakko Pohjoismäki, Jonas J. Astrin, Astrid Böhne, Chiara Bortoluzzi, Elena Bužan, Javier del Campo, Claudio Ciofi, Camilla B. Di-Nizo, Pradeep K. Divakar, 2026, pregledni znanstveni članek Opis: High-quality reference genome assemblies have become essential for deepening our understanding of biodiversity, yet obtaining them for many species remains surprisingly challenging. Drawing on experiences from the European Reference Genome Atlas (ERGA) community, we focus on permit and sample-handling procedures leading up to nucleic acid sequencing, covering tasks such as ensuring ethical and legal compliance, verifying accurate species identification, maintaining sample integrity during transport, and isolating high-quality DNA or nuclei. While many of the challenges and solutions we discuss are broadly relevant, our regulatory and logistical examples are primarily from Europe. By synthesising practical guidance, we highlight the crucial importance of taxonomic expertise, proper vouchering and biobanking, rigorous cold-chain management or alternative preservation methods, and emphasise adherence to packaging and shipping requirements for biological materials. We showcase examples spanning diverse regions, taxa and source materials, which underscore the importance of context-specific strategies and internationally harmonised protocols, particularly for metadata reporting. Our recommendations aim to support both small-scale projects and large initiatives, directing collective efforts to facilitate efficient sampling, vouchering and sample processing for future genomic studies. Ključne besede: permits, samples, genomics Objavljeno v RUP: 21.01.2026; Ogledov: 389; Prenosov: 8
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5. Biodiversity genomics research practices require harmonising to meet stakeholder needs in conservationElena Bužan, Christian de Guttry, Chiara Bortoluzzi, Nathaniel R. Street, Kay Lucek, Anna Rosling, Lino Ometto, Alice Mouton, Luísa S. Marins, María José Ruiz-López, José Melo-Ferreira, Elisabet Ottosson, Camila J. Mazzoni, Robert M. Waterhouse, 2025, izvirni znanstveni članek Opis: Biodiversity resilience relies on genetic diversity, which sustains the evolutionary potential of organisms in dynamic ecosystems. Genomics is a powerful tool for accurately estimating genetic diversity across genomes of species and populations. However, integration of genomic data into conservation efforts faces challenges due to the heterogeneity of approaches employed. Establishing common sets of standards for genomic data production and analysis is essential to consistently interpret results and clearly communicate outcomes to stakeholders. While the European Reference Genome Atlas (ERGA) community has contributed significantly to the standardisation of reference genome methodologies in synergy with other initiatives, there is now an urgent need to extend these principles to downstream analyses. ERGA aims to build on its experience to help establish harmonised approaches in applied biodiversity genomics research, aligned with ongoing efforts to define standardised metrics for measuring and reporting genetic diversity. Establishing consensus on best practices for genome-wide data generation methods and applications will substantially increase accuracy, interpretability, and comparability, together with enhanced stakeholder capacities. By identifying key opportunities and challenges, as well as conducting preliminary stakeholder mapping and examining case studies, the goal is to build an inclusive framework that ensures the relevance and widespread adoption of these best practices: fostering trust and confidence in genomics research practices to meet stakeholder needs in biodiversity conservation. We call upon the broader research community to join efforts in establishing these approaches, recognising the importance of participation of end-users, to foster the integration of genomic data into the toolkit for measuring and reporting genetic diversity. Ključne besede: best practices, biodiversity genomics, genome-wide genetic diversity, stakeholder engagement, standardisation, whole genome resequencing data Objavljeno v RUP: 16.01.2026; Ogledov: 427; Prenosov: 2
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6. Effective cryopreservation of post mortem-collected roe deer gametes by evaluation of post-thaw oocyte and sperm characteristics and in vitro fertilizationAnna Justyna Korzekwa, Elena Bužan, Boštjan Pokorny, Gulsum Ummu Boztepe, Marek Lecewicz, Władysław Kordan, 2025, izvirni znanstveni članek Opis: The aim was to evaluate the effectiveness of semen cryopreservation and oocyte vitrification in roe deer as a potential method of gamete preservation for endangered deer species. Sperm were isolated from the cauda epididymis of fourteen bucks (n = 14). The motility measure (CASA) and morphology of fresh semen (FS) and frozen–thawed semen (TS) were compared. A hyaluronic binding assay was used to distinguish between mature FS spermatozoa expressing hyaluronan receptors and immature FS lacking these receptors, and the mitochondrial membrane potential (MMP) in TS was determined (flow cytometry). A Sperm–Hyaluronan Binding Assay (HBA) showed a viability rate of 61.9% in FS and 78.2% in TS. Oocytes received from eight does (n = 8) underwent a viability test and vitrification, and fresh oocytes from the other eight does (n = 8) were fertilized with TS and embryos were cultured until the blastocyst stage. The number of isolated oocytes, cumulus–oocyte complexes (COCs), cleaved embryos, and expanded blastocysts was evaluated. Higher percentages of morphological factors (acrosome, head, midpiece, and tail shape) were observed in FS compared to TS, whereas the motility and progressive movement were greater in TS (p ≤ 0.001). The viability was 50.5% and MMP was 40.6% in TS. A total of 311 oocytes were collected and from 150 COCs and 125 blastocysts developed. The viability of thawed oocytes after vitrification was 81%. The viability of vitrified oocytes and cryopreserved sperm confirmed the effectiveness of freezing protocols and highlights the potential for their implementation in other deer species. Ključne besede: roe deer, semen, oocytes, fertilization Objavljeno v RUP: 13.10.2025; Ogledov: 474; Prenosov: 14
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7. The role of community science in DNA-based biodiversity monitoringCarolina Corrales, Karolina Bacela-Spychalska, Elena Bužan, Torbjørn Ekrem, Sónia Ferreira, William Goodall-Copestake, Elaine van Ommen Kloeke, Peter M. Hollingsworth, Sarah J. Bourlat, 2025, izvirni znanstveni članek Opis: The mutual interest in nature by the general public and scientists has led to many collaborations, past and present. Community science shows great potential for monitoring species occurrences and distributions, especially in combination with scalable and (semi)-automated methods such as DNA-based monitoring, helping to obtain data from a broader geographic and temporal range than would be possible by the scientific community alone. Here, we present an overview of the complementarity between community science and DNA-based biomonitoring through examples from ongoing projects. The involvement of hobby experts is particularly crucial for building up the necessary species reference databases that enable DNA-based monitoring. Based on this overview, we identify some key points related to learning opportunities and participant recognition to maximise the success, impact and benefit of community participants in DNA-based monitoring. Ključne besede: eDNA, community science, genetics Objavljeno v RUP: 13.10.2025; Ogledov: 448; Prenosov: 2
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8. Piškurji Slovenije : pregled in primerjava metod izlovaLuka Ložar, 2025, diplomsko delo Ključne besede: elektroribolov, Eudontomyzon vladykovi, Lampetra zanandreai, metoda ZZRS, monitoring, piškurji, posplošeni linearni mešani model, učinkovitost izlova, vzorčenje, diplomske naloge Objavljeno v RUP: 07.10.2025; Ogledov: 566; Prenosov: 0 |
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10. Navigating COP16’s digital sequence information outcomes : what researchers need to do in practiceMelania Muñoz-García, Amber Hartman Scholz, 2025, drugi znanstveni članki Opis: The UN Convention on Biological Diversity adopted new rules for sharing benefits from publicly available genetic sequence data, also known as digital sequence information (DSI). In this Opinion, the authors describe the key elements researchers need to be aware of, address real-life questions, and explain the practical implications of these rules for research and development. Ključne besede: COP16, digital sequence information, sequences, research Objavljeno v RUP: 26.09.2025; Ogledov: 794; Prenosov: 8
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