1. First EST-SSRs of Helichrysum italicum (Roth) G. Don (Asteraceae) revealed insights into the genetic diversity and population structure in CorsicaPetra Gabrovšek, Matjaž Hladnik, Dunja Bandelj, Zala Jenko Pražnikar, Saša Kenig, Félix Tomi, Marc Gibernau, Slavko Brana, Alenka Baruca Arbeiter, 2025, izvirni znanstveni članek Opis: Helichrysum italicum (Roth) G. Don (Asteraceae) is a valuable medicinal and aromatic plant native to a variety of habitats across the Mediterranean region. However, genetic studies of this morphologically diverse species have been limited by the scarcity of species-specific DNA markers. To address this limitation, we generated the first de novo transcriptome assembly comprising 24,806 transcripts from young shoots containing leaves and flowers, developed EST-SSR markers, and evaluated their utility in population genetic analysis. Seventy-eight primer pairs were designed, of which 23 showed successful amplification, polymorphism, and transferability to Helichrysum litoreum Guss. and Helichrysum arenarium (L.) Moench. A subset of 12 EST-SSRs was used to genotype 270 individuals from 12 natural populations of H. italicum in Corsica (France), along with one outgroup population from Croatia. The polymorphic information content ranged from 0.250 to 0.796, and Shannon’s information index ranged from 0.588 to 1.843, indicating the markers’ suitability for popu- lation genetic studies. Analysis of molecular variance revealed that 15% of the total genetic variation was attributable to differences among populations. Discriminant analysis of principal components and Bayesian clustering in STRUCTURE identified distinct popu- lation clusters corresponding to geographic locations. Notably, the southernmost coastal populations were clearly differentiated from the others.
Ključne besede: Helichrysum italicum, immortelle, de novo transcriptome, genic microsatellite markers, EST-SSRs, population study, Corsica, Mediterranean Basin
Objavljeno v RUP: 17.12.2025; Ogledov: 173; Prenosov: 6
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2. Population genetic structure of European wildcats inhabiting the area between the Dinaric Alps and the Scardo-Pindic mountainsFelicita Urzi, Nikica Šprem, Hubert Potočnik, Magda Sindičić, Dean Konjević, Duško Ćirović, Andrea Rezić, Luka Duniš, Dime Melovski, Elena Bužan, 2021, izvirni znanstveni članek Ključne besede: genetic variation, microsatellite markers, hybridisation, wildcat
Objavljeno v RUP: 18.10.2021; Ogledov: 3381; Prenosov: 56
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3. Analysis of genetic diversity in Croatian fig (Ficus carica L.) germplasm using SSR markersDanijela Poljuha, Ines Kralj, Marin Krapac, Tatjana Klepo, Mira Radunić, Frane Strikić, Tim Weber, Sezai Ercişli, Alenka Baruca Arbeiter, Matjaž Hladnik, Dunja Bandelj, 2021, objavljeni znanstveni prispevek na konferenci Ključne besede: Ficus carica, germplasm, national collections, genetic diversity, Croatia, microsatellite
Objavljeno v RUP: 18.10.2021; Ogledov: 3255; Prenosov: 35
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4. Genetic structure of wildcat (Felis silvestris) populations in the area between the Dinaric and Scardo-Pindic MountainsFelicita Urzi, Nikica Šprem, Hubert Potočnik, Magda Sindičić, Dean Konjević, Duško Ćirović, Andrea Rezić, Luka Duniš, Dime Melovski, Elena Bužan, 2021, objavljeni povzetek znanstvenega prispevka na konferenci Ključne besede: endangered felid, genetic structure, hybridization, microsatellite
Objavljeno v RUP: 06.05.2021; Ogledov: 2859; Prenosov: 15
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5. Genetics as a useful tool for understanding relatedness, movements and social behaviour in wild boarAja Bončina, Elena Bužan, Boštjan Pokorny, Laura Iacolina, 2021, objavljeni povzetek znanstvenega prispevka na konferenci Ključne besede: microsatellite, relatedness, spatial behaviour, social structure, wild boar
Objavljeno v RUP: 04.02.2021; Ogledov: 2613; Prenosov: 20
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6. Development and characterization of expressed SSRs in olive (Olea europaea L.)Alenka Baruca Arbeiter, Jernej Jakše, Dunja Bandelj, 2015, objavljeni povzetek znanstvenega prispevka na konferenci Ključne besede: oljka, Olea europaea L., izraženo nukleotidno zaporedje, genski mikrosatelitski markerji, olive, Olea europaea L., expressed sequence tag, EST, genic microsatellite markers
Objavljeno v RUP: 15.10.2015; Ogledov: 5085; Prenosov: 74
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