1. Surface colonization of Aureobasidium pullulans : a multimodal microscopy study toward living coating developmentIhab Malat, Anja Černoša, Anna Malgorzata Sandak, 2026, izvirni znanstveni članek Opis: Recent advances in engineered living materials (ELMs), which integrate living cells into functional structural and protective systems, have accelerated interest in understanding microbial surface interactions at fundamental scales. In this context, Aureobasidium pullulans, a polymorphic and polyextremotolerant black yeast fungus, has emerged as a promising candidate for diverse biotechnological applications, including engineered living coatings. However, its microscale surface colonization dynamics remain insufficiently characterized, limiting predictive control over colonization of various surfaces. Complementary optical approaches, including VHX digital imaging, fluorescence observation, and quantitative fluorescence measurements, were used to characterize temporal colonization of fungus on plastic coverslips and pine wood, representing two contrasting substrates used in the building sector. On plastic substrates, initial attachment involved dispersed cells that rapidly proliferated and merged into a continuous layer. Quantitative fluorescence revealed a significant increase in signal from Day 1 to Day 3 (from 25,328 to 42,510 RFU; P = 5.4 × 10⁻⁴) followed by a decrease by Day 6 (10,555 RFU). This decline may be associated with reduced dye penetration into the compact, melanized matrix rather than a reduction in biomass. On wood, colonization followed the native fiber orientation and progressed into cohesive multicellular structures. The results provide a new understanding of substrate-dependent colonization of A. pullulans and highlight methodological limitations in quantifying biomass on porous, heterogeneous materials. The multimodal microscopy framework established a robust comparative platform for analysing fungal-material interactions and enabled the rational development of fungal-based living coatings for protective and functional applications.
Ključne besede: Aureobasidium pullulans, surface colonization, plastic, pine wood, living coating
Objavljeno v RUP: 06.05.2026; Ogledov: 142; Prenosov: 9
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2. Assessment of marine litter interaction in cetaceans stranded along the Italian coastline and the Adriatic SeaGuido Pietroluongo, Matteo Baini, Cristina Casalone, Giorgia Corazzola, Martina Đuras, Stefano Gavaudan, Federica Giorda, Tilen Genov, Silva Rubini, Sandro Mazzariol, 2025, izvirni znanstveni članek Ključne besede: marine litter, plastic ingestion, cetaceans, post-mortem, Italy, Adriatic sea, marine strategy framework directive, conservation
Objavljeno v RUP: 23.11.2025; Ogledov: 582; Prenosov: 2
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6. Ingestion of marine debris by loggerhead sea turtles, Caretta caretta, in the Adriatic SeaBojan Lazar, Romana Gračan, 2011, izvirni znanstveni članek Opis: We examined the occurrence of marine debris in the gastrointestinal tract of 54 loggerhead sea turtles (Caretta caretta) found stranded or incidentally captured dead by fisheries in the Adriatic Sea, with a curved carapace length of 25.0-79.2 cm. Marine debris was present in 35.2% of turtles and included soft plastic, ropes, Styrofoam and monofilament lines found in 68.4%, 42.1%, 15.8% and 5.3% of loggerheads that have ingested debris, respectively. The dry mass of debris per turtle was low, ranging from <0.01 to 0.71 g, and the ingestion was not significantly affected by sex or body siye (all p > 0.05). Marine debris averaged 2.2 +- 8.0% of dry mass of gut content, with a maximum of 35% found in a juvenile turtle that most likely died due to debris ingestion. Considering the relatively high occurrence of debris intake and possible sub-lethal effects of even small quantities of marine debris, this can be an additional factor of concern for loggerheads in the Adriatic Sea
Ključne besede: Sea turtles, Caretta caretta, marine debris, plastic, pollution, Adriatic Sea
Objavljeno v RUP: 15.10.2013; Ogledov: 6460; Prenosov: 151
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