41. Human beta-defensin-3 producing cells in septic implant looseningJaakko Levón, Ahmed Al-Samadi, Zygmunt Mackiewicz, Andrej Cör, Rihard Trebše, Eero Waris, Yrjö T. Konttinen, 2015, izvirni znanstveni članek Opis: Abstract Human ß-defensin-3 (hBD-3) has been found in synovial fluid and later in periprosthetic tissues in septic joint implant loosening. The aim of the present study was to identify its cellular sources. Tissue samples from 12 patients were analyzed. A fully automatic Leica BOND MAX staining robot was used. Affinity-purified rabbit anti-human hBD-3 IgG was applied in a two-layer horse radish peroxidase/anti-rabbit-labeled polymer method. Double immunofluorescence of hBD3 together with CD68, CD31, heat shock protein 47 (HSP47) and mast cell tryptase (MCT) staining was done. Human BD-3 was found in monocyte/macrophage-like cells, vascular endothelial cells and fibroblasts-like cells, but was weakly expressed in foreign body giant cells and negative in neutrophils. Human BD-3 was found in CD68 and CD31 immunoreactive cells, whereas HSP47 and MCT positive cells were hBD-3 negative. Immunostaining of hBD-3 was strong in some tissue areas but weak or absent in others. Monocyte/macrophages and endothelial cells were established in this study as the major cellular sources of hBD-3 in septic loosening, but fibroblasts and foreign body giant cells can also contribute to its production. The heterogeneous topological staining of hBD-3 suggests local regulation, possibly by bacterial products, damage-associated molecular patterns and cytokines. The results explain the increased synovial fluid/tissue concentrations of hBD-3 in septic loosening.
Ključne besede: beta-defensin 3, septic loosening, arthroplasty
Objavljeno v RUP: 14.10.2015; Ogledov: 3559; Prenosov: 318
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42. Breaking down taboos concerning sexuality among the elderlyDoroteja Rebec, Igor Karnjuš, Sabina Ličen, Katarina Babnik, 2015, samostojni znanstveni sestavek ali poglavje v monografski publikaciji Ključne besede: tabuji, spolnost, seksualnost, starejši, zdravstveni delavci, domovi starejših, sexuality, elderly, nursing homes, attitudes, nursing care professions
Objavljeno v RUP: 14.10.2015; Ogledov: 5090; Prenosov: 379
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43. Dejavniki poklicnega odločanja v zdravstveni negi in v prehranskem svetovanju - dietetikiKatarina Babnik, Mojca Stubelj, Sabina Ličen, Tamara Poklar Vatovec, Patrik Pucer, Tamara Štemberger Kolnik, Boštjan Žvanut, 2015, samostojni znanstveni sestavek ali poglavje v monografski publikaciji Ključne besede: kariera, poklicno odločanje, dietetika, prehransko svetovanje, zdravstvena nega
Objavljeno v RUP: 14.10.2015; Ogledov: 5302; Prenosov: 106
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44. A balanced scorecard-based model for evaluating e-learning and conventional pedagogical activities in nursingTomaž Hovelja, Damjan Vavpotič, Boštjan Žvanut, 2015, izvirni znanstveni članek Opis: The evaluation of e-learning and conventional pedagogical activities in nursing programmes has focused either on a single pedagogical activity or the entire curriculum, and only on students or teachers' perspective. The goal of this study was to design and test a novel approach for evaluation of e-learning and conventional pedagogical activities that considers students', teachers' and managers' perspectives. A case study of the proposed approach was performed at a publicly funded nursing faculty with Slovenian and Italian students from 2009 to 2012. The case study was combined with focus group discussions, interviews, direct observation and survey. The proposed approach allows management to compare the value of different pedagogical activities through the students', teachers' and managers' perspectives. The approach proved useful in the evaluation of pedagogical activities and provided valid arguments for long-term pedagogical process improvement
Ključne besede: evaluation, curriculum, cost-effectivness, e-learning
Objavljeno v RUP: 14.10.2015; Ogledov: 4725; Prenosov: 232
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47. Endoglin (CD105) silencing mediated by shRNA under the control of Endothelin-1 promoter for targeted gene therapy of MelanomaNataša Tešić, Urška Kamenšek, Gregor Serša, Simona Kranjc Brezar, Monika Savarin, Urša Lampreht Tratar, Véronique Préat, Gaëlle Vandermeulen, Miha Butinar, Boris Turk, Maja Čemažar, 2015, izvirni znanstveni članek Opis: Endoglin (CD105), a transforming growth factor (TGF)-% coreceptor, and endothelin-1, a vasoconstrictor peptide, are both overexpressed in tumor endothelial and melanoma cells. Their targeting is therefore a promising therapeutic approach for melanoma tumors. The aim of our study was to construct a eukaryotic expression plasmid encoding the shRNA molecules against CD105 under the control of endothelin-1 promoter and to evaluate its therapeutic potential both in vitro in murine B16F10-luc melanoma and SVEC4-10 endothelial cells and in vivo in mice bearing highly metastatic B16F10-luc tumors. Plasmid encoding shRNA against CD105 under the control of the constitutive U6 promoter was used as a control. We demonstrated the antiproliferative and antiangiogenic effects of both plasmids in SVEC4-10 cells, as well as a moderate antitumor and pronounced antimetastatic effect in B16F10-luc tumors in vivo. Our results provide evidence that targeting melanoma with shRNA molecules against CD105 under the control of endothelin-1 promoter is a feasible and effective treatment, especially for the reduction of metastatic spread.
Ključne besede: melanoma, electroporation, endoglin (CD105), endothelin-1, gene therapy
Objavljeno v RUP: 14.10.2015; Ogledov: 4940; Prenosov: 134
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