1. A virus based vaccine combined with IL12 gene therapy eradicates aggressive melanomaNuša Brišar, Katja Šuster, Simona Kranjc Brezar, Andrej Cör, 2025, original scientific article Abstract: Our study introduces a novel bacteriophage-based vaccine strategy and evaluates its antitumor efficacy, both as a standalone therapy and in combination with gene electrotransfer (GET) of interleukin-12 (IL-12) plasmids. Using phage display technology, we produced engineered M13 bacteriophages expressing tumour peptides MAGE-A1, gp100, or MART-1/MELAN-A on the surface of the capsid. The therapeutic potential of bacteriophage vaccination alone or in combination with GET IL-12 was tested in vivo in a mouse malignant melanoma model. Response to treatment was further characterized by histological and immunohistochemical analyses of tumour tissue. No negative side effects were observed during treatment in mice. Engineered bacteriophage therapy significantly delayed tumour growth. GET IL-12 contributed to the therapeutic effect of engineered bacteriophages and increased tumour growth delay. Both therapies had a synergistic effect and led to complete responses in 30% of cases. Histological and immunohistochemical analyses have shown that both bacteriophage monotherapy and, especially in combination with GET IL-12, activate the immune system and increase the proportion of necrosis and the infiltration of macrophages, CD4 + and CD8 + T lymphocytes in tumours. For the first time, a cocktail of three engineered M13 bacteriophages displaying different melanoma-associated antigens with intratumoral IL-12 gene electrotransfer were applied, demonstrating a synergistic therapeutic effect in a highly aggressive melanoma model. Nanotechnological approaches, such as the use of genetically engineered bacteriophages, offer promising new avenues for the development of anti-tumour vaccines.
Keywords: immunotherapy, bacteriophages, bacteriophage display technology, bacteriophage vaccine, interleukin, gene electrotransfer, malignant melanoma
Published in RUP: 16.06.2025; Views: 97; Downloads: 2
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2. Slovenski dan dietetike : prva znanstvena konferenca z mednarodno udeležbo, Izola, 25. oktober 20122012, proceedings of peer-reviewed scientific conference contributions (domestic conferences) Keywords: debelost, presnova, prebavila, bolezni, prehranska podpora, zdrava prehrana, aditivi, mikrohranila, javno zdravje, živila, označevanje, kardiovaskularne bolezni, ajda, hranilna vrednost, presnovne bolezni, zborniki, CD-ROM
Published in RUP: 09.11.2021; Views: 1764; Downloads: 42
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4. Pomen avtologne transfuzije pri operacijah kolka ali kolena : diplomska nalogaTomaž Kozjek, 2011, undergraduate thesis Keywords: transfuziologija, avtotransfuzija, sklepne proteze, zdravstvena nega, kolenske proteze, kolčne proteze, ortopedske operacije, endoproteze, ortopedska kirurgija, diplomske naloge
Published in RUP: 18.03.2019; Views: 2975; Downloads: 75
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5. Pomen zdravstvene vzgoje pri prehranjevalnih navadah starostnikov v Mestni občini Koper : diplomska nalogaDijana Letić, 2011, undergraduate thesis Keywords: starostniki, zdrava prehrana, prehranjevalne navade, zdravstvena vzgoja, izobrazba, družbeni status, socialni status, kvantitativne metode, diplomske naloge
Published in RUP: 18.03.2019; Views: 3227; Downloads: 53
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