1. Surface colonization of Aureobasidium pullulans : a multimodal microscopy study toward living coating developmentIhab Malat, Anja Černoša, Anna Malgorzata Sandak, 2026, izvirni znanstveni članek Opis: Recent advances in engineered living materials (ELMs), which integrate living cells into functional structural and protective systems, have accelerated interest in understanding microbial surface interactions at fundamental scales. In this context, Aureobasidium pullulans, a polymorphic and polyextremotolerant black yeast fungus, has emerged as a promising candidate for diverse biotechnological applications, including engineered living coatings. However, its microscale surface colonization dynamics remain insufficiently characterized, limiting predictive control over colonization of various surfaces. Complementary optical approaches, including VHX digital imaging, fluorescence observation, and quantitative fluorescence measurements, were used to characterize temporal colonization of fungus on plastic coverslips and pine wood, representing two contrasting substrates used in the building sector. On plastic substrates, initial attachment involved dispersed cells that rapidly proliferated and merged into a continuous layer. Quantitative fluorescence revealed a significant increase in signal from Day 1 to Day 3 (from 25,328 to 42,510 RFU; P = 5.4 × 10⁻⁴) followed by a decrease by Day 6 (10,555 RFU). This decline may be associated with reduced dye penetration into the compact, melanized matrix rather than a reduction in biomass. On wood, colonization followed the native fiber orientation and progressed into cohesive multicellular structures. The results provide a new understanding of substrate-dependent colonization of A. pullulans and highlight methodological limitations in quantifying biomass on porous, heterogeneous materials. The multimodal microscopy framework established a robust comparative platform for analysing fungal-material interactions and enabled the rational development of fungal-based living coatings for protective and functional applications.
Ključne besede: Aureobasidium pullulans, surface colonization, plastic, pine wood, living coating
Objavljeno v RUP: 06.05.2026; Ogledov: 156; Prenosov: 9
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2. BIM-based framework for estimating service life duration of wood construction elements under environmental exposureRichard Acquah, Jonas Niklewski, Anna Malgorzata Sandak, Jakub Michal Sandak, 2026, izvirni znanstveni članek Opis: As the construction industry adopts more data-driven and sustainable practices, Building Information Modelling (BIM) is increasingly used for energy analysis, lifecycle management, and maintenance planning. However, its integration with service life performance analysis especially for wood, a material susceptible to environmental degradation remains limited. This study presents a BIM-based framework that incorporates wood-specific parameters such as environmental exposure, material properties, and design detailing to estimate the service life of wood construction elements. The framework is structured using ISO 15,686–4 principles and implemented through custom Industry Foundation Classes (IFC) property sets. A Revit plugin developed in C# integrates the model into the BIM environment. A case study of a wooden playhouse is used as a proof-of-concept to demonstrates the framework’s ability to evaluate the impact of design, material, and environmental factors on service life. The results confirm that the framework supports service life prediction and enhances decision-making for wood-based construction. Twenty-one-month observations align with predicted results. However, long-term (2–9 year) predictions remain unvalidated, requiring extended monitoring. By integration of the service life prediction for fungal decay into BIM it offers a practical and scalable tool to support service life–informed design and planning for wood structures.
Ključne besede: service life prediction, wood material durability, environmental exposure, wood 28 construction, sustainable construction, building information modelling (BIM)
Objavljeno v RUP: 24.11.2025; Ogledov: 643; Prenosov: 10
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4. Photochemical behavior of colloidal lignin particles under controlled UV exposure : balancing self-stabilization and degradationJulia Tomasich, Rene Alexander Herrera Diaz, Anna Malgorzata Sandak, Michael Harasek, Stefan Beisl, Oihana Gordobil, 2025, izvirni znanstveni članek Opis: Colloidal Lignin Particles (CLPs), with their polyphenolic structure, are promising sustainable alternatives to chemical UV filters. This study investigates the photochemical behavior of CLPs under ultraviolet irradiation synthetized from five different technical raw lignins (Alkali, Organosolv, two Enzymatic Hydrolyzed and Softwood Kraft Lignin) via solvent-shift procedure. The suspensions were irradiated using a self-developed UV-pen set-up and a commercially available UV chamber, enabling controlled UV exposure over time. Variations in the physicochemical properties of irradiated colloidal lignin particles were characterized by Multi-Angle Dynamic Light Scattering (MADLS), Fourier Transform Infrared Spectroscopy (FTIR) coupled with chemometrics, UV–Vis spectroscopy, and High-Performance Size Exclusion Chromatography (HP-SEC). CLPs exhibited comparable responses after 14 days of UV exposure, with alterations in particle chemistry, particularly in the formation of new functional groups and molecular rearrangement. Moreover, the photochemical stability of CLPs was found to be highly dependent on both their concentration and the nature of the dispersion medium (aqueous, hexane-based, and solid-state). At higher concentrations (1.8 mg/mL), CLPs demonstrated effective self-stabilization, while lower concentrations led to rapid degradation, resulting in the disappearance of particles and the formation of low-molecular-weight fragments. Additionally, Minimal photochemical changes were observed in non-aqueous or solid-state environments due to restricted molecular mobility. These findings provide valuable insights into the degradation pathways and stability across different environmental contexts, which can contribute to understanding the potential environmental impact of CLPs in aquatic ecosystems.
Ključne besede: biobased materials, lignin colloidal particles, photodegradation, UV-irradiation, spectroscopy
Objavljeno v RUP: 09.09.2025; Ogledov: 772; Prenosov: 6
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5. Auditory emotional design in multimedia learning : educational videos on wood as a building materialNežka Sajinčič, 2024, doktorska disertacija Ključne besede: multimedia learning, auditory emotional design, instructor’s voice, emotional tone, background music, same-language subtitles, learning in a foreign language, wood as a building material
Objavljeno v RUP: 14.10.2024; Ogledov: 1800; Prenosov: 76
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6. Feasibility of portable NIR spectrometer for quality assurance in glue-laminated timber productionJakub Michal Sandak, Peter Niemz, Andreas Hänsel, Juana Mai, Anna Malgorzata Sandak, 2021, izvirni znanstveni članek Ključne besede: near infrared spectroscopy, glue-laminated timber, glulam, delamination, quality assurance
Objavljeno v RUP: 18.10.2021; Ogledov: 4829; Prenosov: 27
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7. An exploratory study of consumers' knowledge and attitudes about lignin-based sunscreens and bio-based skincare productsNežka Sajinčič, Oihana Gordobil, Amy Noel Simmons, Anna Malgorzata Sandak, 2021, izvirni znanstveni članek Ključne besede: attitude, bio-based ingredients, environmental concern, green cosmetics, health consciousness, human factors, knowledge, lignin sunscreen, preference, skincare
Objavljeno v RUP: 18.10.2021; Ogledov: 4091; Prenosov: 58
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8. Special Issue "Wood modification: characterization, modelling, and applications"Anna Malgorzata Sandak, Jakub Michal Sandak, 2021, predgovor, uvodnik, spremna beseda Ključne besede: wood modification, characterization, modelling, applications
Objavljeno v RUP: 30.07.2021; Ogledov: 3103; Prenosov: 35
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9. Bioinspired living coating system in service : evaluation of the wood protected with biofinish during one-year natural weatheringFaksawat Poohphajai, Jakub Michal Sandak, Michael Sailer, Lauri Rautkari, Tiina Belt, Anna Malgorzata Sandak, 2021, izvirni znanstveni članek Ključne besede: natural weathering, bio-based coating, service life performance, aesthetics, living fungal cells, bioinspired materials design
Objavljeno v RUP: 24.06.2021; Ogledov: 2783; Prenosov: 77
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10. Growth of xerophilic fungi on model paint samples on glass and wooden supports under low humidity conditionsJanez Kosel, Jakub Michal Sandak, Anna Malgorzata Sandak, Lea Legan, Klara Retko, Maša Kavčič, Črtomir Tavzes, Miklós Ferenz Krész, Polonca Ropret, 2021, objavljeni povzetek znanstvenega prispevka na konferenci Ključne besede: Aspergillus, Penicillium, xerophilic fungi, pigments
Objavljeno v RUP: 24.06.2021; Ogledov: 2871; Prenosov: 74
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