1. Processing and mounting phlebotomine sand flies : a consensus guidelineFano José Randrianambinintsoa, Laure Augendre, Jorian Prudhomme, Jean-Philippe Martinet, Mathieu Loyer, Nalia Mekarnia, Hocine Kerkoub, Farzana Khan Perveen, Antoine Huguenin, Vladimir Ivović, 2026, pregledni znanstveni članek Opis: This article provides a comprehensive guide for the processing and mounting of phlebotomine sand fly specimens, which is crucial for species identification and pathogen detection and isolation. It discusses a range of techniques suitable for both field and laboratory settings. The guide includes detailed instructions on sand fly collec- tion, handling, covering, and euthanasia (recommending dry freezing or CO2 over chemicals) as well as conservation strategies, such as cold storage and preservation in ethanol. The quality of preparation of certain anatomical structures (genital organs, head and wings) is essential for their proper microscopic observation and is described in this work. The article also presents detailed sample processing, including the clearing process with agents such as potassium hydrox- ide then Marc-André solution. The mounting process compares different media, emphasizing their optical properties and preservation potential. Hoyer fluid (also known as chloral gum) is recommended for quick observation, particularly for spermathecae, due to its clarity, although it is not suitable for long-term storage. Other media discussed include polyvinyl alcohol, EuparalÒ (for limited water tolerance), and Canada balsam (a hydrocarbon-soluble medium), with the latter two offering long-term preservation capabilities. Innovative molecular biology approaches such as DNA sequencing and MALDI-ToF, which require particular attention to sample processing, are also addressed. Furthermore, short video clips illustrating various mounting techniques as well as translations in many different languages are provided, allowing the guideline to reach the diverse needs and expectations of the global scientific community.
Ključne besede: mounting, Phlebotomine sand fly, Hoyer fluid, Marc-André solution, Chloral gum, Polyvinyl alcohol, Euparal®, Canada balsam, Leishmania isolation, field conditions, culture, dissection, molecular biology, MALDI-ToF, type-specimens
Objavljeno v RUP: 03.04.2026; Ogledov: 557; Prenosov: 9
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2. Qualitative content analysis of COVID-19’s role in suicide attempts leading to hospital careMartina Mravlja, Anthony Pisani R., Annamarie Bailey, Nicola Meda, Alexandre Paim-Diaz, Kristina Zurich, Kenneth Conner, 2025, izvirni znanstveni članek Opis: Introduction: The impact of the COVID-19 pandemic on suicide risk has been documented during the acute phase, but less is known about people who attempted suicide during the post-acute period. This study investigates how adults who attempted suicide during the post-acute pandemic period (2021–2023) understood COVID-19’s role in their attempt. Method: We analyzed interview data from 329 adults (59% female; 41% male), enrolled following a recent suicide attempt between 2021 and 2023. Participants were asked about the general impact of COVID-19 on their lives and then specifically about whether stress related to COVID-19 was a primary reason for their attempt or contributed to their suicidal thoughts. Results: When asked about their recent attempt, 11% of participants identified stress related to COVID-19 as the primary reason for their attempt, and an additional 23% indicated it contributed to their suicidal thoughts. When describing general impacts, participants reported effects across multiple domains: social isolation, physical health concerns, mental health impacts, and economic effects. Discussion: The attribution of suicide attempts to COVID-19-related stress during the post-acute period highlights the extended impact of public health crises on vulnerable individuals. These findings emphasize the need for sustained, integrated medical and mental healthcare following such crises.
Ključne besede: suicide, COVID-19, pandemic mental health, loneliness, isolation, healthcare, stress, prevention
Objavljeno v RUP: 17.12.2025; Ogledov: 661; Prenosov: 5
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3. Beasley, LeRoy B., Kang, Kyung-Tae, Song, Seok-Zun: Isolation numbers of integer matrices and their preservers. (English summary). - Bull. Korean Math. Soc. 57 (2020), no. 3, 535-545Marko Orel, 2021, recenzija, prikaz knjige, kritika Ključne besede: preserver problems, matrices with nonnegative coefficients, isolation number
Objavljeno v RUP: 06.05.2021; Ogledov: 2511; Prenosov: 10
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4. Dokaz in identifikacija bakterije Borrelia burgdorferi sensu lato v klopih obalno-kraške in primorsko-notranjske regije : magistrsko deloJana Šušnjar, 2020, magistrsko delo Ključne besede: klopi, Borrelia burgdorferi sensu lato, Lajmska borelioza, Ixodes ricinus, kultivacija in osamitev, PCR v realnem času, ticks, Borrelia burgdorferi sensu lato, Lyme borreliosis, Ixodes ricinus, cultivation and isolation, real time PCR
Objavljeno v RUP: 02.10.2020; Ogledov: 6179; Prenosov: 43
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