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Neural agrin changes the electrical properties of developing human skeletal muscle cells
Mihaela Jurdana, Guido Fumagalli, Zoran Grubič, Paola Lorenzon, Tomaž Marš, Marina Sciancalepore, 2009, izvirni znanstveni članek

Opis: Recent investigations suggest that the effects of neural agrin might not be limited to neuromuscular junction formation and maintenance and that other aspects of muscle development might be promoted by agrin. Here we tested the hypothesis that agrin induces a change in the excitability properties in primary cultures of non-innervated human myotubes. Electrical membrane properties of human myotubes were recorded using the whole-cell patch-clamp technique. Cell incubation with recombinant chick neural agrin (1 nM) led to amore negative membrane resting potential. Addition of strophanthidin, a blocker of the Na(+)žK(+) ATPase, depolarized agrin-treated myotubes stronger than control, indicating, in the presence of agrin, a higher contribution of the Na(+)žK(+) ATPase in establishing the resting membrane potential. Indeed, larger amounts of both the alpha1 and the alpha2 isoforms of the Na(+)žK(+) ATPase protein were expressed in agrin-treated cells. A slight but significantdown-regulation of functional apamin-sensitive K(+) channels was observed after agrin treatment. These results indicate that neural agrin mightact as a trophic factor promoting the maturation of membrane electrical properties during differentiation, confirming the role of agrin as a general promoter of muscle development.
Najdeno v: osebi
Ključne besede: agrin, myogenesis, electrical membrane properties, skeletal muscle, Na+/K+ pump
Objavljeno: 15.10.2013; Ogledov: 1672; Prenosov: 83
URL Polno besedilo (0,00 KB)
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Effect of ionizing radiation on human skeletal muscle precursor cells
Tomaž Marš, Maja Čemažar, Mihaela Jurdana, Katarina Pegan, 2013, objavljeni znanstveni prispevek na konferenci

Opis: Background. Long term effects of different doses of ionizing radiation on human skeletal muscle myoblast proliferation, cytokine signalling and stress response capacity were studied in primary cell cultures.Materials and methods. Human skeletal muscle myoblasts obtained from muscle biopsies were cultured and irradiated with a Darpac 2000 X-ray unit at doses of 4, 6 and 8 Gy. Acute effects of radiation were studied by interleukin - 6 (IL-6) release and stress response detected by the heat shock protein (HSP) level, while long term effects were followed by proliferation capacity and cell death.Results. Compared with non-irradiated control and cells treated with inhibitor of cell proliferation Ara C, myoblast proliferation decreased 72 h post-irradiation, this effect was more pronounced with increasing doses. Post-irradiation myoblast survival determined by measurement of released LDH enzyme activity revealed increased activity after exposure to irradiation. The acute response of myoblasts to lower doses of irradiation (4 and 6 Gy) was decreased secretion of constitutive IL-6. Higher doses of irradiation triggered a stress response in myoblasts, determined by increased levels of stress markers (HSPs 27 and 70).Conclusions. Our results show that myoblasts are sensitive to irradiation in terms of their proliferation capacity and capacity to secret IL-6. Since myoblast proliferation and differentiation are a key stage in muscle regeneration, this effect of irradiation needs to be taken in account, particularly in certain clinical conditions.
Najdeno v: osebi
Ključne besede: myoblasts, irradiation, proliferation, interleukin 6, muscle regeneration, apoptosis
Objavljeno: 30.12.2015; Ogledov: 1894; Prenosov: 106
URL Polno besedilo (0,00 KB)

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