| Naslov: | Intraspecific geographical variability of Phlebotomus perniciosus assessed by MALDI-TOF MS protein profiling |
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| Avtorji: | ID Dvořák, Vít (Avtor)
ID Maia, Carla (Avtor)
ID Marmé, Rafael (Avtor)
ID Risueño, José (Avtor)
ID Pérez-Cutillas, Pedro (Avtor)
ID Berriatua, Eduardo (Avtor)
ID Sevila, Julie (Avtor)
ID Mekarnia, Nalia (Avtor)
ID Prudhomme, Jorian (Avtor)
ID Randriananambinintsoa, Fano José (Avtor)
ID Ivović, Vladimir (Avtor)
ID Adam, Katja (Avtor) |
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| Datoteke: |  RAZ_Dvořak_Vit_2026.pdf (944,26 KB)
MD5: ED916D712F4FC8451C6C3EFACF8CAE47
 https://link.springer.com/article/10.1186/s13071-026-07354-9
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Članek v reviji |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | FAMNIT - Fakulteta za matematiko, naravoslovje in informacijske tehnologije
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| Opis: | Background Matrix‑assisted laser desorption/ionization time‑of‑flight mass spectrometry (MALDI‑TOF MS) pro‑ tein profiling has emerged over the last decade as a method of choice for species identification of many medically important arthropods. However, the influence of intraspecific variability on the performance of this popular technique has seldom been tested. This study provides the first standardized comparison of different geographical popula‑ tions of Phlebotomus perniciosus, a vector of Leishmania infantum and Toscana virus in the western Mediterranean, by MALDI‑TOF MS protein profiling. Methods Phlebotomus perniciosus males were collected in five countries (Portugal, Spain, France, Italy, Croa‑ tia) that represent most of its distribution in Europe. All samples were trapped, stored and processed according to a highly standardized protocol to avoid effects other than geographical origin on their protein spectra acquired by MALDI‑TOF MS protein profiling. The obtained protein spectra were compared with laboratory‑reared specimens of Ph. perniciosus. Results Twenty‑two analysed specimens from five geographical populations provided protein spectra that were highly similar, species‑specific and clustering according to their quality. No grouping according to geographical origin was observed, and the protein spectra of field‑collected specimens showed similar composition and complexity to spectra from Ph. perniciosus laboratory colony‑reared in captivity for several decades. Conclusions Our findings demonstrate that in samples of a same sex, with the same collection method and storage time, MALDI‑TOF MS protein profiling does not reflect the geographical origin of analysed specimens, confirming the value of this technique for high‑fidelity and reproducible species identification of sand flies regardless of their geographical origin. |
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| Ključne besede: | intraspecific geographical variability, Phlebotomus perniciosus, MALDI-TOF |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 23.03.2026 |
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| Leto izida: | 2026 |
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| Št. strani: | str. 1-8 |
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| Številčenje: | Vol. 19, article no. ǂ197 |
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| PID: | 20.500.12556/RUP-23186  |
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| UDK: | 595.772.1 |
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| ISSN pri članku: | 1756-3305 |
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| COBISS.SI-ID: | 282921731 |
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| Datum objave v RUP: | 29.06.2026 |
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| Število ogledov: | 27 |
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| Število prenosov: | 2 |
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| Metapodatki: |    |
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