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Naslov:Endoglin (CD105) silencing mediated by shRNA under the control of Endothelin-1 promoter for targeted gene therapy of Melanoma
Avtorji:ID Tešić, Nataša (Avtor)
ID Kamenšek, Urška (Avtor)
ID Serša, Gregor (Avtor)
ID Kranjc Brezar, Simona (Avtor)
ID Savarin, Monika (Avtor)
ID Lampreht Tratar, Urša (Avtor)
ID Préat, Véronique (Avtor)
ID Vandermeulen, Gaëlle (Avtor)
ID Butinar, Miha (Avtor)
ID Turk, Boris (Avtor)
ID Čemažar, Maja (Avtor)
Datoteke:URL http://www.nature.com/mtna/journal/v4/n5/full/mtna201512a.html
 
Jezik:Angleški jezik
Vrsta gradiva:Delo ni kategorizirano
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FVZ - Fakulteta za vede o zdravju
Opis:Endoglin (CD105), a transforming growth factor (TGF)-% coreceptor, and endothelin-1, a vasoconstrictor peptide, are both overexpressed in tumor endothelial and melanoma cells. Their targeting is therefore a promising therapeutic approach for melanoma tumors. The aim of our study was to construct a eukaryotic expression plasmid encoding the shRNA molecules against CD105 under the control of endothelin-1 promoter and to evaluate its therapeutic potential both in vitro in murine B16F10-luc melanoma and SVEC4-10 endothelial cells and in vivo in mice bearing highly metastatic B16F10-luc tumors. Plasmid encoding shRNA against CD105 under the control of the constitutive U6 promoter was used as a control. We demonstrated the antiproliferative and antiangiogenic effects of both plasmids in SVEC4-10 cells, as well as a moderate antitumor and pronounced antimetastatic effect in B16F10-luc tumors in vivo. Our results provide evidence that targeting melanoma with shRNA molecules against CD105 under the control of endothelin-1 promoter is a feasible and effective treatment, especially for the reduction of metastatic spread.
Ključne besede:melanoma, electroporation, endoglin (CD105), endothelin-1, gene therapy
Leto izida:2015
Številčenje:No. 4
PID:20.500.12556/RUP-7057 Povezava se odpre v novem oknu
ISSN:2162-2531
UDK:577.2
DOI:10.1038/mtna.2015.12 Povezava se odpre v novem oknu
COBISS.SI-ID:1537399748 Povezava se odpre v novem oknu
Datum objave v RUP:14.10.2015
Število ogledov:4733
Število prenosov:134
Metapodatki:XML DC-XML DC-RDF
:
TEŠIĆ, Nataša, KAMENŠEK, Urška, SERŠA, Gregor, KRANJC BREZAR, Simona, SAVARIN, Monika, LAMPREHT TRATAR, Urša, PRÉAT, Véronique, VANDERMEULEN, Gaëlle, BUTINAR, Miha, TURK, Boris in ČEMAŽAR, Maja, 2015, Endoglin (CD105) silencing mediated by shRNA under the control of Endothelin-1 promoter for targeted gene therapy of Melanoma. [na spletu]. 2015. Vol. 4. [Dostopano 13 april 2025]. DOI 10.1038/mtna.2015.12. Pridobljeno s: http://www.nature.com/mtna/journal/v4/n5/full/mtna201512a.html
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